The Effect of 17% Ethylenediaminetetraacetic Acid And 10% Citric Acid Solutions with Manual and Ultrasonic Agitation on Smear Layer Removal and Intraradicular Dentine Erosion: a Scanning Electron Microscope Evaluation Efeito das Soluções de EDTA 17% e ácido Cítrico 10% Através da Agitação Manual e Ultrassônica na Remoção do Smear Layer: Análise em Microscópio Eletrônico de Varredura

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Alessandro Rodrigo Maggioni
Helena Rosa Campos Rabang
Brenda Paula Figueiredo de Almeida Gomes
Luiza Gonçalves Roma
Isabel Coelho Gomes Camões

Resumo

Este estudo ex vivo avaliou a eficácia de diferentes protocolos de irrigação final na remoção da smear layer e na erosão da dentina intrarradicular.Trinta e cinco caninos humanos extraídos foram ins- trumentados e divididos aleatoriamente, de acordo com as técnicas de irrigação final utilizadas, em 7 grupos: 1 (ED3M) e 3 (ED3US), EDTA a 17% por 3 minutos com agitação manual e ultrassônica, respectivamente; 2 (CA30M) e 4 (CA30US), ácido cítrico a 10% por 30 segundos com agitação manual e ultrassônica, respectiva- mente; 5 (CA3M) e 6 (CA3US), ácido cítrico a 10% por 3 minutos com agitação manual e ultrassônica, respectivamente; e 7 (Na3), NaOCl a 5,25% por 3 minutos sem agitação (controle). Todas as amostras foram irrigadas com NaOCl a 5,25%, divididas longitudi- nalmente e examinadas em microscopia eletrônica de varredura (MEV) nos terços apical, médio e coronal. Os dados foram analisa- dos pelos testes de Kruskal-Wallis e Mann-Whitney. A eficácia do EDTA 17% e do ácido cítrico 10% na remoção da smear layer foi significativamente maior que NaOCl 5,25% (controle). Não houve diferenças significativas entre os protocolos finais de irrigação na remoção da smear layer ou nos efeitos erosivos. Entretanto, ao comparar os terços dos grupos 1 (ED3M) e 2 (CA30M), a menor remoção da camada de smear layer e erosão foram observadas no terço apical, mas o procedimento do grupo 4 (CA30US) foi mais eficaz que 1 (ED3M) (p = 0,0004), 2 (CA30M) (p = 0,0018) ou 3


(ED3US) (p = 0,0003) na remoção da smear layer no terço apical.


Concluiu-se que os protocolos utilizados neste estudo foram se- melhantes na remoção da smear layer e efeitos erosivos.

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Como Citar
Rodrigo Maggioni, A., Rosa Campos Rabang, H., Paula Figueiredo de Almeida Gomes, B., Gonçalves Roma, L., & Coelho Gomes Camões, I. (2020). The Effect of 17% Ethylenediaminetetraacetic Acid And 10% Citric Acid Solutions with Manual and Ultrasonic Agitation on Smear Layer Removal and Intraradicular Dentine Erosion: a Scanning Electron Microscope Evaluation. Revista Naval De Odontologia, 47(I), 7-13. Recuperado de https://jmksistemas.com.br/revista/index.php/odontoclinica/article/view/92
Seção
Artigos Originais
Biografia do Autor

Alessandro Rodrigo Maggioni

Dental Department, Endodontic clinic, Naval Dental Center, Brazilian Navy, Rio de Janeiro, Brazil

Helena Rosa Campos Rabang, Brazilian Navy, , Naval Dental Center

 

Capitão de Mar e Guerra (CD) ex - diretora da Odontoclínica Central da Marinha, Marinha do Brasil, Rio de Janeiro, Brasil

Faculdade de Odontologia de Campinas, Piracicaba, São Paulo, Brasil

 

Brenda Paula Figueiredo de Almeida Gomes, Marinha do Brasil

Faculdade de Odontologia, Campinas, Piracicaba - SP, Brasil.

Luiza Gonçalves Roma, Marinha do Brasil, Odontoclínica Central da Marinha

Clínica de Endodontia, Odontoclínica Central da Marinha, Marinha do Brasil, Rio de Janeiro, Brasil

Isabel Coelho Gomes Camões, Universidade Federal Fluminense - UFF - Niterói - RJ - Brasil

Faculdade de Odontologia, Universidade Federal Fluminense,UFF, Niterói - RJ, Brasil

Referências

1. McComb D, Smith DC. A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod. 1975 Jul 1(7):238-42.
2. Goldman LB, Goldman M, Kronman JH, Lin PS. The efficacy

of several irrigating solutions for endodontics: a scanning electron microscopic study. Oral Surg Oral Med Oral Pathol. 1981 Aug 52(2):197-204.
3. Mader CL, Baumgartner JC, Peters DD. Scanning electron microscopic investigation of the smeared layer on root canal walls. J Endod. 1984 Oct 10(10):477-83.
4. Virdee SS, Seymour DW, Farnell D, Bhamra G, Bhakta S. Efficacy of irrigant activation techniques in removing intracanal smear layer and debris from mature permanent teeth: a systematic review and meta-analysis. Int Endod J. 2018 Jun 51(6):605-621.
5. Czonstkowsky M, Wilson EG, Holstein FA. The smear layer in endodontics. Dent Clin North Am. 1990 Jan 34(1):13-25.
6. Cobankara FK, Adanr N, Belli S. Evaluation of the influence of smear layer on the apical and coronal sealing ability of two sealers. J Endod. 2004 Jun 30(6):406-9.
7. White RR, Goldman M, Lin PS.The influence of the smeared layer upon dentinal tubule penetration by plastic filling materials. J Endod. 1984 Dec 10(12):558-62.
8. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res. 1981 Aug 89(4):321-8.
9. Bystrom A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J. 1985 Jan 18(1):35-40.
10. Sen BH, Wesselink PR, Türkün M. The smear layer: a phenomenon in root canal therapy. Int Endod J. 1995 May 28(3):141-8.
11. Hülsmann M, Heckendorff M, Lennon A. Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J. 2003 Dec 36(12):810-30.
12. De-Deus G, Paciornik S, Mauricio MH. Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine. Int Endod J. 2006 May 39(5):401-7.
13. Sceiza MF, Daniel RL, Santos EM, Jaeger MM. Cytotoxic effects of 10% citric acid and EDTA-T used as root canal irrigants: an in vitro analysis. J Endod. 2001 Dec 27(12):741-3.
14. De-Deus G, Paciornik S, Pinho Mauricio MH, Prioli R. Real-time atomic force microscopy of root dentine during demineralization when subjected to chelating agents. Int Endod J. 2006 Sep 39(9):683-92.
15. Deari S, Mohn D, Zehnder M. Dentine decalcification and smear layer removal by different ethylenediaminetetraacetic acid and 1-hydroxyethane-1,1-diphosphonic acid species. Int Endod J. 2019 Feb 52(2):237-243.
16. Yamada RS, Armas A, Goldman M, Lin PS. A scanning electron microscopic comparison of a high volume final flush with several irrigating solutions: Part 3. J Endod. 1983 Apr 9(4):137-42.
17. Zehnder M. Root canal irrigants. J Endod. 2006 May 32(5):389-98.

18. Calt S, Serper A. Time-dependent effects of EDTA on dentin structures. J Endod. 2002 Jan 28(1):17-9.
19. Niu W, Yoshioka T, Kobayashi C, Suda H. A scanning electron microscopic study of dentinal erosion by final irrigation with EDTA and NaOCl solutions. Int Endod J. 2002 Nov 35(11):934-9.
20. Lopes HP; Elias CN; Estrela C; Toniasso S. Mechanical stirring of smear layer removal: Influence of the chelating agent (EDTA). Bras Endod J 1996; 1: 52-5.
21. Martin H. Ultrasonic disinfection of the root canal. Oral Surg Oral Med Oral Pathol. 1976 Jul 42(1):92-9.
22. Roy RA,Ahmad M, Crum LA. Physical mechanisms governing the hydrodynamic response of an oscillating ultrasonic file. Int Endod J. 1994 Jul 27(4):197-207.
23. Garberoglio R, Becce C. Smear layer removal by root canal irrigants. A comparative scanning electron microscopic study. Oral Surg Oral Med Oral Pathol. 1994 Sep 78(3):359-67. 24.Torabinejad M, Khademi AA, Babagoli J, Cho Y, Johnson WB, Bozhilov K, et al. A new solution for the removal of the smear layer. J Endod. 2003 Mar 29(3):170-5.
25. Hülsmann M; Peters O; Dummer PMH. Mechanical preparation of root canals: Shaping goals, techniques and means. Endod Topics. 2005 Mar 10(1):30-76.
26. Estrela C, Estrela CR, Barbin EL, Spanó JC, Marchesan MA, Pécora JD. Mechanism of action of sodium hypochlorite. Braz Dent J. 2002;13(2):113-7.
27. Pérez-Heredia M, Ferrer-Luque CM, González-Rodríguez MP. The effectiveness of different acid irrigating solutions in root canal cleaning after hand and rotary instrumentation. J Endod. 2006 Oct 32(10):993-7.
28. Di Lenarda R, Cadenaro M, Sbaizero O. Effectiveness of 1 mol L-1 citric acid and 15% EDTA irrigation on smear layer removal. Int Endod J. 2000 Jan 33(1):46-52.
29. Scelza MF, Pierro V, Scelza P, Pereira M. Effect of three

different time periods of irrigation with EDTA-T, EDTA, and citric acid on smear layer removal. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004 Oct 98(4):499-503.
30. Reis C, De-Deus G, Leal F, Azevedo E, Coutinho-Filho T, Paciornik S. Strong effect on dentin after the use of high concentrations of citric acid: na assessment with co-site optical microscopy and ESEM. Dent Mater. 2008 Dec 24(12):1608-15.
31. Ramachandran N, Podar R, Singh S, Kulkarni G, Dadu S. Effect of ultrasonic activation on calcium ion quantification, smear layer removal, and canal cleaning efficacy of demineralizing irrigants. J Conserv Dent. 2018 Sep-Oct;21(5):551-556.
32. Mello I, Kammerer BA, Yoshimoto D, Macedo MC, Antoniazzi JH. Influence of final rinse technique on ability of ethylenediaminetetraacetic acid of removing smear layer. J Endod. 2010 Mar 36(3):512-4.
33. Lui JN, Kuah HG, Chen NN. Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer. J Endod. 2007 Apr 33(4):472-5.
34. Kuah HG, Lui JN, Tseng PS, Chen NN. The effect of EDTA with and without ultrasonics on removal of the smear layer. J Endod. 2009 Mar 35(3):393-6.
35. Tinaz AC, Karadag LS, Alaçam T, Mihçioglu T. Evaluation of the smear layer removal effectiveness of EDTA using two techniques: an SEM study. J Contemp Dent Pract. 2006 Feb 15;7(1):9-16.
36. Saber Sel-D, Hashem AA. Efficacy of different final irriga- tion activation techniques on smear layer removal. J Endod. 2011 Sep 37(9):1272-5.
37. Sedgley CM, Nagel AC, Hall D, Applegate B. Influence of irrigant needle depth in removing bioluminescent bacteria ino- culated into instrumented root canals using real-time imaging in vitro. Int Endod J. 2005 Feb 38(2):97-104.
38. Calt S, Serper A. Smear layer removal by EGTA. J Endod. 2000 Aug 26(8):459-61.